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PCR Basics

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Joseph Ross

00:00 Introduction to polymerase chain reaction (PCR)
01:04 Primers
02:34 Primers interact with templates
03:07 Three steps of PCR
03:55 Denature
04:55 Anneal
06:15 Extension; DNA polymerase
07:57 Positions of primers define the length of the amplicon
09:42 "Big picture" view of PCR cycling over time
14:18 Uses of PCR in the study of genetics
14:57 Details of a setting up a PCR experiment
20:27 PCR is sensitive to contamination

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